Journal: Journal of cachexia, sarcopenia and muscle

Article Title: Transcriptional Co-Activator With PDZ Binding Motif (TAZ) Inhibits Dexamethasone-Induced Muscle Atrophy via mTOR Signalling.

doi: 10.1002/jcsm.13790

Figure Lengend Snippet: FIGURE 4 | TAZ stimulates mTOR signalling. (A) Left: C2C12 myotubes were treated with either a vehicle [DEX(−)] or dexamethasone [DEX(+)]. The levels of Rheb, RhebL1, phospho-p70 S6K (p-p70 S6K), p70 S6K and vinculin were analysed by immunoblotting. Right: The protein levels depict- ed in the left panel were quantified (n = 3). (B) Left: TAZ- and TAZ4SA-overexpressing C2C12 myotubes were treated with either a vehicle [DEX(−)] or dexamethasone [DEX(+)]. The levels of RhebL1, p-p70 S6K, p70 S6K and α-tubulin were analysed by immunoblotting. Right: The protein levels shown in the left panel were quantified (n = 3). (C) Left: The control (Con), TAZ and TAZ4SA AAV viruses were introduced into the GA muscle of mice. Following dexamethasone administration, gastrocnemius muscle was isolated, and the levels of RhebL1, p-p70 S6K and p70 S6K were anal- ysed by immunoblotting. Right: The protein levels of the left panel were quantified (n = 3). α-Tubulin in Figure 2F was used for the quantification of RhebL1. The data are presented as the mean ± SD for Panels (A) and (B) and as the mean ± SEM for Panel (C). Statistical analysis was performed using appropriate tests: Panel (A) was analysed using one-tailed t-test, Panel (B) was analysed using two-way ANOVA followed by Holm–Sidak's multiple comparisons test, and Panel (C) was analysed using one-way ANOVA followed by Tukey's multiple comparisons test. Exact p values are provided in the graph, and non-significant results are not explicitly indicated.

Article Snippet: Antibodies against TAZ/YAP (8418), Rheb (13879), Vinculin (13901), p- p70 S6K (9234) and p70 S6K (2708) were purchased from Cell Signaling Technology.

Techniques: Western Blot, Control, Isolation, One-tailed Test